Please use this identifier to cite or link to this item: https://doi.org/10.1046/j.1365-2761.2000.00229.x
Title: Enhancement of protective immunity in blue gourami, Trichogaster trichopterus (Pallas), against Aeromonas hydrophila and Vibrio anguillarum by A. hydrophila major adhesin
Authors: Fang, H.M.
Ling, K.C.
Ge, R. 
Sin, Y.M. 
Issue Date: 2000
Citation: Fang, H.M., Ling, K.C., Ge, R., Sin, Y.M. (2000). Enhancement of protective immunity in blue gourami, Trichogaster trichopterus (Pallas), against Aeromonas hydrophila and Vibrio anguillarum by A. hydrophila major adhesin. Journal of Fish Diseases 23 (2) : 137-145. ScholarBank@NUS Repository. https://doi.org/10.1046/j.1365-2761.2000.00229.x
Abstract: Blue gourami, Trichogaster trichopterus (Pallas), were intraperitoneally immunized with major adhesin, a 43 kDa OMP protein isolated from fish Aeromonas hydrophila, in the presence of Freund's complete adjuvant (FCA). Three weeks later, a booster injection of adhesin without FCA was administered. Control group fish were similarly treated with phosphate-buffered saline (PBS) and FCA. Results showed that anti-adhesin serum obtained from fish after booster immunization exhibited very strong ability in agglutinating bacterial cells. Although this antiserum had no bactericidal effect, it could significantly inhibit serologically different strains of A. hydrophila from invading EPC (Epithelioma papillosum of carp) cells in vitro. In addition, the proliferative response of head kidney leucocytes of these immune fish was significantly increased as compared to that of the control. The results also showed that the major adhesin could provide significant protective immunity to fish against the challenge by homologous and heterologous strains of A. hydrophila and one virulent strain of Vibrio anguillarum.
Source Title: Journal of Fish Diseases
URI: http://scholarbank.nus.edu.sg/handle/10635/100583
ISSN: 01407775
DOI: 10.1046/j.1365-2761.2000.00229.x
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