Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100552
Title: Effects of peritoneal injection of NH4HCO3 on nitrogen excretion and metabolism in the swamp eel Monopterus albus - Increased ammonia excretion with an induction of glutamine synthetase activity
Authors: Lim, C.K.
Chew, S.F.
Tay, A.S.L.
Ip, Y.K. 
Issue Date: 1-Apr-2004
Citation: Lim, C.K.,Chew, S.F.,Tay, A.S.L.,Ip, Y.K. (2004-04-01). Effects of peritoneal injection of NH4HCO3 on nitrogen excretion and metabolism in the swamp eel Monopterus albus - Increased ammonia excretion with an induction of glutamine synthetase activity. Journal of Experimental Zoology Part A: Comparative Experimental Biology 301 (4) : 324-333. ScholarBank@NUS Repository.
Abstract: Monopterus albus has to deal with high environmental ammonia concentrations during dry seasons and agricultural fertilization in rice fields. In this study, NH4HCO3 (10 μmol per g fish) was injected into the peritoneal cavity of M. albus, raising the level of ammonia in the body, in order to elucidate the strategies involved in defense against the toxicity of exogenous ammonia. During the subsequent 24 h after NH4HCO 3 injection, there was a significant increase in the ammonia excretion rate, which indicates that the main strategy adopted by M. albus was to remove the majority of the exogenous ammonia through enhanced ammonia excretion. Exogenous ammonia was not detoxified into urea for excretion or accumulation. Six hours post-injection of NH4HCO3, ammonia contents in the tissues built up significantly, especially in the brain, which suggests that M. albus had high tolerance of ammonia toxicity at the cellular and sub-cellular levels. By hour 12 post-injection, there were significant increases in the activities of glutamine synthetase in the muscle, liver, and gut, accompanied by significant increases in glutamine contents in the muscle and the liver. There was also a significant increase in the glutamine content in the brain at hour 6 post-injection of NH4HCO3. These results confirm the capability of M. albus to detoxify ammonia through glutamine synthesis. Overall, injection of NH4HCO3 had only minor effects on the contents of FAAs, other than glutamine, in tissues of M. albus because the majority (70%) of the injected ammonia was excreted within the 24-h period. © 2004 Wiley-Liss, Inc.
Source Title: Journal of Experimental Zoology Part A: Comparative Experimental Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/100552
ISSN: 0022104X
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