Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.diff.2009.05.009
Title: DTA-mediated targeted ablation revealed differential interdependence of endocrine cell lineages in early development of zebrafish pancreas
Authors: Li, Z. 
Korzh, V. 
Gong, Z. 
Keywords: Diphtheria toxin gene A chain
Glucagon
Insulin
Pancreas
Somatostatin
Zebrafish
Issue Date: Nov-2009
Citation: Li, Z., Korzh, V., Gong, Z. (2009-11). DTA-mediated targeted ablation revealed differential interdependence of endocrine cell lineages in early development of zebrafish pancreas. Differentiation 78 (4) : 241-252. ScholarBank@NUS Repository. https://doi.org/10.1016/j.diff.2009.05.009
Abstract: Cell lineage analysis is critical in understanding the relationship between progenitors and differentiated cells as well as the mechanism underlying the process of differentiation. In order to study the zebrafish endocrine pancreas cell lineage, transgenic expression of diphtheria toxin gene A chain (DTA) under two cell type-specific promoters derived from the insulin (ins) and somatostatin2 (sst2) genes was used to ablate the two types of endocrine cells: insulin-producing β-cells and somatostatin-producing δ-cells, respectively. We found that ablation of β-cells resulted in a reduction of not only β-cells but also glucagon-producing α-cells; in contrast, δ-cells were largely unaffected. Ablation of δ-cells led to reduction of all three types of endocrine cells: α-, β-, and δ. Interestingly, α-cells were more profoundly affected in both β- and δ-cell ablations and were frequently reduced together with β- and δ-cells. By taking advantage of Tg(ins:gfp) and Tg(sst2:gfp) lines, we also monitored the changes of different types of endocrine cells in vivo after ablation and found that both β- and δ-cell populations significantly recovered by 3 dpf after their ablation and it seemed that δ-cells had a better capability of recovery than β-cells. Thus, our current observations indicated differential interdependence of these three cell lineages. The development of zebrafish α-cells, but not δ-cells, is dependent on β-cells, while the development of both α- and β-cells is dependent on δ-cells. In contrast, the development of δ-cells was independent of β-cells. © 2009 International Society of Differentiation.
Source Title: Differentiation
URI: http://scholarbank.nus.edu.sg/handle/10635/100508
ISSN: 03014681
DOI: 10.1016/j.diff.2009.05.009
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

7
checked on Jun 11, 2019

WEB OF SCIENCETM
Citations

7
checked on Jun 11, 2019

Page view(s)

68
checked on May 25, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.