Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100474
Title: Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa
Authors: Teo, W.
Lakshmanan, P. 
Kumar, P. 
Goh, C.-J. 
Swarup, S. 
Keywords: Aminoethoxyvinylglycine
Rooting
Shoot regeneration
Issue Date: Oct-1997
Citation: Teo, W.,Lakshmanan, P.,Kumar, P.,Goh, C.-J.,Swarup, S. (1997-10). Direct shoot formation and plant regeneration from cotyledon explants of rapid-cycling Brassica rapa. In Vitro Cellular and Developmental Biology - Plant 33 (4) : 288-292. ScholarBank@NUS Repository.
Abstract: An in vitro culture system for direct shoot regeneration from cotyledon explants of rapid-cycling Brassica rapa was developed. Cotyledons from 3-d-old seedlings, when cultured on Murashige and Skoog (MS) medium supplemented with 20 μM N6-benzyladenine (BA) and 2 μM α-naphthaleneacetic acid (NAA), regenerated shoots directly at a frequency of 20%. The addition of 2 μM aminoethoxyvinylglycine (AVG) to this medium increased shoot regeneration to 33%, but silver nitrate drastically inhibited shoot regeneration. Shoot regeneration occurred directly, at the petiolar cut ends of cotyledonary explants, between 10 to 17 d in culture. The highest percentage of regeneration (33%) was obtained from 3-d-old seedlings. NAA was the most effective auxin for root induction and development, with 49% of shoots producing roots after 2 wk on medium containing 1.0 μM NAA. Regenerated plantlets were grown to maturity in pots containing peat moss and vermiculite (1:1). These plants were morphologically normal and fertile. With this protocol, over 100 independently derived, flowering R0 plants were obtained from 40 regenerating cotyledonary explants within 40 d after culture initiation.
Source Title: In Vitro Cellular and Developmental Biology - Plant
URI: http://scholarbank.nus.edu.sg/handle/10635/100474
ISSN: 10545476
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.