Please use this identifier to cite or link to this item: https://doi.org/10.1242/dev.020255
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dc.titleDirect interaction of AGL24 and SOC1 integrates flowering signals in Arabidopsis
dc.contributor.authorLiu, C.
dc.contributor.authorChen, H.
dc.contributor.authorER HONG LING
dc.contributor.authorSoo, H.M.
dc.contributor.authorKumar, P.P.
dc.contributor.authorHan, J.-H.
dc.contributor.authorLiou, Y.C.
dc.contributor.authorYu, H.
dc.date.accessioned2014-10-27T08:26:13Z
dc.date.available2014-10-27T08:26:13Z
dc.date.issued2008-04
dc.identifier.citationLiu, C., Chen, H., ER HONG LING, Soo, H.M., Kumar, P.P., Han, J.-H., Liou, Y.C., Yu, H. (2008-04). Direct interaction of AGL24 and SOC1 integrates flowering signals in Arabidopsis. Development 135 (8) : 1481-1491. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.020255
dc.identifier.issn09501991
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100471
dc.description.abstractDuring the transition from vegetative to reproductive growth, the shoot meristem of flowering plants acquires the inflorescence identity to generate flowers rather than vegetative tissues. An important regulator that promotes the inflorescence identity in Arabidopsis is AGAMOUS-LIKE 24 (AGL24), a MADS-box transcription factor. Using a functional estradiol-inducible system in combination with microarray analysis, we identified AGL24-induced genes, including SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1), a floral pathway integrator. Chromatin immunoprecipitation (ChIP) analysis of a functional AGL24-6HA-tagged line revealed in vivo binding of AGL24-6HA to the regulatory region of SOC1. Mutagenesis of the AGL24 binding site in the SOC1 promoter decreased Prosoc1:GUS expression and compromised SOC1 function in promoting flowering. Our results show that SOC1 is one of the direct targets of AGL24, and that SOC1 expression is upregulated by AGL24 at the shoot apex at the floral transitional stage. ChIP assay using a functional SOC1-9myc-tagged line and promoter mutagenesis analysis also revealed in vivo binding of SOC1-9myc to the regulatory regions of AGL24 and upregulation of AGL24 at the shoot apex by SOC1. Furthermore, we found that as in other flowering genetic pathways, the effect of gibberellins on flowering under short-day conditions was mediated by the interaction between AGL24 and SOC1. These observations suggest that during floral transition, a positive-feedback loop conferred by direct transcriptional regulation between AGL24 and SOC1 at the shoot apex integrates flowering signals.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1242/dev.020255
dc.sourceScopus
dc.subjectArabidopsis
dc.subjectChromatin immunoprecipitation
dc.subjectFlowering time
dc.subjectGibberellin
dc.subjectMADS-box transcription factor
dc.subjectTranscriptional regulation
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1242/dev.020255
dc.description.sourcetitleDevelopment
dc.description.volume135
dc.description.issue8
dc.description.page1481-1491
dc.description.codenDEVPE
dc.identifier.isiut000254917700010
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