Please use this identifier to cite or link to this item: https://doi.org/10.1038/nprot.2010.104
Title: Derivation and characterization of haploid embryonic stem cell cultures in medaka fish
Authors: Yi, M. 
Hong, N.
Hong, Y. 
Issue Date: Aug-2010
Citation: Yi, M., Hong, N., Hong, Y. (2010-08). Derivation and characterization of haploid embryonic stem cell cultures in medaka fish. Nature Protocols 5 (8) : 1418-1430. ScholarBank@NUS Repository. https://doi.org/10.1038/nprot.2010.104
Abstract: Embryonic stem (ES) cells are pluripotent cells capable of differentiation into various cell types. Haploid ES cells elegantly combine the advantages of haploidy and pluripotency and offer a unique in vitro system for genetic analyses of molecular, cellular and developmental events in various cell lineages. Our recent success in generating haploid ES cell lines from gynogenetic embryos of the medaka fish suggests that haploidy can support ES cell derivation and maintenance in a vertebrate. In this study, we present a step-by-step protocol for derivation and characterization of medaka haploid ES cells. We have used this procedure to produce three haploid ES cell lines from five primary cultures. It takes about 15 weeks to generate stable cultures, 5g-8 weeks to obtain pure haploid cells and 5-6 weeks to characterize ES cells in vitro and in vivo. © 2010 Nature America, Inc. All rights reserved.
Source Title: Nature Protocols
URI: http://scholarbank.nus.edu.sg/handle/10635/100418
ISSN: 17542189
DOI: 10.1038/nprot.2010.104
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