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Title: Cubic membranes: A structure-based design for DNA uptake
Authors: Almsherqi, Z.
Hyde, S.
Ramachandran, M. 
Deng, Y.
Keywords: Cubic membranes
Cubic phases
DNA delivery
Fluorescence microscopy
Transmission electron microscopy
Issue Date: 6-Sep-2008
Citation: Almsherqi, Z., Hyde, S., Ramachandran, M., Deng, Y. (2008-09-06). Cubic membranes: A structure-based design for DNA uptake. Journal of the Royal Society Interface 5 (26) : 1023-1029. ScholarBank@NUS Repository.
Abstract: Cubic membranes are soft three-dimensional crystals found within cell organelles in a variety of living systems, despite the aphorism of Fedorov: 'crystallization is death'. They consist of multi-bilayer lipid-protein stacks, folded onto anticlastic surfaces that resemble triply periodic minimal surfaces, forming highly swollen crystalline sponges. Although cubic membranes have been observed in numerous cell types and under different pathophysiological conditions, knowledge about the formation and potential function(s) of non-lamellar, cubic structures in biological systems is scarce. We report that mitochondria with this cubic membrane organization isolated from starved amoeba Chaos carolinense interact sufficiently with short segments of phosphorothioate oligonucleotides (PS-ODNs) to give significant ODNs uptake. ODNs condensed within the convoluted channels of cubic membrane by an unknown passive targeting mechanism. Moreover, the interaction between ODNs and cubic membrane is sufficient to retard electrophoretic mobility of the ODN component in the gel matrix. These ODN-cubic membrane complexes are readily internalized within the cytoplasm of cultured mammalian cells. Transmission electron microscopic analysis confirms ODNs uptake by cubic membranes and internalization of ODN-cubic membrane complexes into the culture cells. Cubic membranes thus may offer a new, potentially benign medium for gene transfection. © 2008 The Royal Society.
Source Title: Journal of the Royal Society Interface
ISSN: 17425689
DOI: 10.1098/rsif.2007.1351
Appears in Collections:Staff Publications

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