Please use this identifier to cite or link to this item:
|Title:||Crystallization and preliminary X-ray analysis of candoxin, a novel reversible neurotoxin from the Malayan krait Bungarus candidus||Authors:||Paaventhan, P.
|Issue Date:||1-Mar-2003||Citation:||Paaventhan, P., Joseph, J.S., Nirthanan, S., Rajaseger, G., Gopalakrishnakone, P., Kini, M.R., Kolatkar, P.R. (2003-03-01). Crystallization and preliminary X-ray analysis of candoxin, a novel reversible neurotoxin from the Malayan krait Bungarus candidus. Acta Crystallographica - Section D Biological Crystallography 59 (3) : 584-586. ScholarBank@NUS Repository. https://doi.org/10.1107/S0907444903001094||Abstract:||Candoxin, a novel three-finger toxin from Bungarus candidus, is a reversible antagonist of muscle (αβγδ) but a poorly reversible antagonist of neuronal α7 nicotinic acetylcholine receptors. It has a molecular weight of 7344 Da, with 66 amino-acid residues including ten half-cystines. The fifth disulfide bridge is located at the tip of loop I (Cys6-Cys11) instead of in loop II as found in other α-neurotoxins. Interestingly, candoxin lacks the segment cyclized by the fifth disulfide bridge at the tip of the middle loop of long-chain neurotoxins, which was reported to be critical for binding to α7 receptors. As a first step to determining its three-dimensional structure, candoxin was crystallized by the hanging-drop vapour-diffusion technique in conditions around 1.5 M sodium chloride, 10%(v/v) ethanol. The crystals formed belonged to the hexagonal system, space group P6222, with unit-cell parameters a = 54.88, b = 54.88, c = 75.54 Å, α = β = 90, γ = 120°, and diffract to a resolution of 1.80 Å. The crystallographic asymmetric unit contains one molecule of candoxin, with an estimated solvent content of 44.6%. Attempts to solve these structures by molecular-replacement methods have not been successful and a heavy-atom derivative search has been initiated.||Source Title:||Acta Crystallographica - Section D Biological Crystallography||URI:||http://scholarbank.nus.edu.sg/handle/10635/100374||ISSN:||09074449||DOI:||10.1107/S0907444903001094|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 25, 2022
WEB OF SCIENCETM
checked on Nov 25, 2022
checked on Nov 24, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.