Please use this identifier to cite or link to this item: https://doi.org/10.1128/JVI.76.23.12320-12324.2002
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dc.titleCovariation in the capsid protein of hibiscus chlorotic ringspot virus induced by serial passaging in a host that restricts movement leads to avirulence in its systemic host
dc.contributor.authorLiang, X.-Z.
dc.contributor.authorLee, B.T.K.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-10-27T08:24:49Z
dc.date.available2014-10-27T08:24:49Z
dc.date.issued2002-12
dc.identifier.citationLiang, X.-Z., Lee, B.T.K., Wong, S.-M. (2002-12). Covariation in the capsid protein of hibiscus chlorotic ringspot virus induced by serial passaging in a host that restricts movement leads to avirulence in its systemic host. Journal of Virology 76 (23) : 12320-12324. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.76.23.12320-12324.2002
dc.identifier.issn0022538X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100347
dc.description.abstractHibiscus chlorotic ringspot virus (HCRSV) from naturally infected Hibiscus rosa-sinensis L. loses virulence in its experimental systemic host Hibiscus cannabinus L. (kenaf) after serial passages in a local lesion host Chenopodium quinoa. Here we report the genetic changes responsible for the loss of virulence at the molecular level. A remarkable covariation of eight site-specific amino acids was found in the HCRSV capsid protein (CP) after serial passages in C. quinoa: Val49→Ile, Ile95→Val, Lys270→Arg, Gly272→Asp, Tyr274→His, Ala311→Asp, Asp334→Ala, and Ala335→Thr. Covariation of at least three of the eight amino acids, Val49, Ile95, and Lys270, caused the virus to become avirulent in kenaf. Interestingly, the nature of the covariation was consistent and reproducible at each serial passage. These data indicate that the nonsynonymous substitutions of amino acids in the HCRSV CP after serial passages in C. quinoa are not likely to be random events but may be due to host-associated positive selection or accelerated genetic drift. The observed interdependence among the three amino acids leading to avirulence in kenaf may have implications for structural or functional relationships in this virus-host interaction.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/JVI.76.23.12320-12324.2002
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1128/JVI.76.23.12320-12324.2002
dc.description.sourcetitleJournal of Virology
dc.description.volume76
dc.description.issue23
dc.description.page12320-12324
dc.description.codenJOVIA
dc.identifier.isiut000179083700053
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