Please use this identifier to cite or link to this item:
Title: Contrasting effects of submergence in light and dark on pyruvate decarboxylase activity in roots of rice lines differing in submergence tolerance
Authors: Mohanty, B.
Ong, B.-L. 
Keywords: Doubled haploid rice lines
Oxygen deficiency
Pyruvate decarboxylase
Rice (Oryza sativa L.)
Submergence tolerance
Issue Date: 1-Jan-2003
Citation: Mohanty, B., Ong, B.-L. (2003-01-01). Contrasting effects of submergence in light and dark on pyruvate decarboxylase activity in roots of rice lines differing in submergence tolerance. Annals of Botany 91 (SPEC. ISS. JAN.) : 291-300. ScholarBank@NUS Repository.
Abstract: Submergence tolerance of 13 doubled haploid lines of rice and their parents (submergence tolerant FR13A and submergence intolerant CT6241) was assessed using 2-week-old seedlings. Plants were scored for leaf senescence and percentage of seedlings that survived up to 15 d submergence, followed by a 12 d recovery period. Seven lines proved to be submergence tolerant, and six relatively intolerant. In all lines, activity of pyruvate decarboxylase (PDC), extracted from the apical 3-5 cm of root axes, decreased by 46-96 % and 38-76 %, respectively, during 5 or 10 d submergence under natural day/night conditions, compared with pre-submergence values (100 %). However, when the enzyme was extracted at night, submergence increased PDC activity of all rice lines (approx. 112 % on average), compared with pre-submergence values (100 %). The stimulating effect of the dark period on PDC activity was reproduced and amplified by submerging rice seedlings for up to 5 d in continuous darkness in water containing sub-ambient concentrations of oxygen (2.3 mg 1-1). Such increased PDC activity was also observed in seedlings exposed to anoxia for 6 h (approx. 6-175 % higher than pre-submergence values). Irrespective of tolerance class, submergence decreased soluble protein concentrations under all conditions and sampling times. No positive correlation was found between PDC activity and tolerance of the various rice lines to submergence. However, PDC activity was slightly higher in submergence intolerant lines, compared with tolerant lines, under both dark submergence and anoxia. Such differences in PDC activity between the two groups of rice lines were not observed when they were submerged under the natural diurnal cycle. Increased PDC activity in roots at night demonstrated a probable incidence of tissue hypoxia or anoxia during submergence during each dark period. © 2003 Annals of Botany Company.
Source Title: Annals of Botany
ISSN: 03057364
DOI: 10.1093/aob/mcf050
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Aug 12, 2019


checked on Jul 10, 2019

Page view(s)

checked on Aug 17, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.