Please use this identifier to cite or link to this item: https://doi.org/10.1128/JVI.74.7.3149-3155.2000
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dc.titleComplete nucleotide sequence and genome organization of hibiscus chlorotic ringspot virus, a new member of the genus Carmovirus: Evidence for the presence and expression of two novel open reading frames
dc.contributor.authorHuang, M.
dc.contributor.authorKoh, D.C.-Y.
dc.contributor.authorWeng, L.-J.
dc.contributor.authorChang, M.-L.
dc.contributor.authorYap, Y.-K.
dc.contributor.authorZhang, L.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-10-27T08:24:22Z
dc.date.available2014-10-27T08:24:22Z
dc.date.issued2000
dc.identifier.citationHuang, M., Koh, D.C.-Y., Weng, L.-J., Chang, M.-L., Yap, Y.-K., Zhang, L., Wong, S.-M. (2000). Complete nucleotide sequence and genome organization of hibiscus chlorotic ringspot virus, a new member of the genus Carmovirus: Evidence for the presence and expression of two novel open reading frames. Journal of Virology 74 (7) : 3149-3155. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.74.7.3149-3155.2000
dc.identifier.issn0022538X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100310
dc.description.abstractThe complete nucleotide sequence of hibiscus chlorotic ringspot virus (HCRSV) was determined. The genomic RNA (gRNA) is 3,911 nucleotides long and has the potential to encode seven viral proteins in the order of 28 (p28), 23 (p23), 81 (p81), 8 (p8), 9 (p9), 38 (p38), and 25 (p25) kDa. Excluding two unique open reading frames (ORFs) encoding p23 and p25, the ORFs encode proteins with high amino acid similarity to those of carmoviruses. In addition to gRNA, two 3'-coterminated subgenomic RNA (sgRNA) species were identified. Full-length cDNA clones derived from gRNA and sgRNA were constructed under the control of a T7 promoter. Both capped and uncapped transcripts derived from the full-length genomic cDNA clone were infectious. In vitro translation and mutagenesis assays confirmed that all the predicted ORFs except the ORF encoding p8 are translatable, and the two novel ORFs (those encoding p23 and p25) may be functionally indispensable for the viral infection cycle. Based on virion morphology and genome organization, we propose that HCRSV be classified as a new member of the genus Carmovirus in family Tombusviridae.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/JVI.74.7.3149-3155.2000
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1128/JVI.74.7.3149-3155.2000
dc.description.sourcetitleJournal of Virology
dc.description.volume74
dc.description.issue7
dc.description.page3149-3155
dc.description.codenJOVIA
dc.identifier.isiut000085974400022
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