Please use this identifier to cite or link to this item: https://doi.org/10.1101/gr.114488.110
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dc.titleCoassembly of REST and its cofactors at sites of gene repression in embryonic stem cells
dc.contributor.authorYu, H.-B.
dc.contributor.authorJohnson, R.
dc.contributor.authorKunarso, G.
dc.contributor.authorStanton, L.W.
dc.date.accessioned2014-10-27T08:24:10Z
dc.date.available2014-10-27T08:24:10Z
dc.date.issued2011-08
dc.identifier.citationYu, H.-B., Johnson, R., Kunarso, G., Stanton, L.W. (2011-08). Coassembly of REST and its cofactors at sites of gene repression in embryonic stem cells. Genome Research 21 (8) : 1284-1293. ScholarBank@NUS Repository. https://doi.org/10.1101/gr.114488.110
dc.identifier.issn10889051
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100292
dc.description.abstractThe differentiation of pluripotent embryonic stem cells is regulated by networks of activating and repressing transcription factors that orchestrate determinate patterns of gene expression. With the recent mapping of target sites for many transcription factors, it has been a conundrum that so few of the genes directly targeted by these factors are transcriptionally responsive to the binding of that factor. To address this, we generated genome-wide maps of the transcriptional repressor REST and five of its corepressors in mouse embryonic stem cells. Combining these binding-site maps with comprehensive gene-expression profiling, we show that REST is functionally heterogeneous. Approximately half of its binding sites apparently are nonfunctional, having weaker binding of REST and low recruitment of corepressors. In contrast, the other sites strongly recruit REST and corepressor complexes with varying numbers of components. Strikingly, the latter sites account for almost all observed gene regulation. These data support a model where productive gene repression by REST requires assembly of a multimeric "repressosome" complex, whereas weak recruitment of REST and its cofactors is insufficient to repress gene expression. © 2011 by Cold Spring Harbor Laboratory Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1101/gr.114488.110
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1101/gr.114488.110
dc.description.sourcetitleGenome Research
dc.description.volume21
dc.description.issue8
dc.description.page1284-1293
dc.description.codenGEREF
dc.identifier.isiut000293335700008
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