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Title: Characterization of polyphenol oxidase from aerial roots of an orchid, Aranda 'Christine 130'
Authors: Ho, K.-K. 
Keywords: Aerial roots
Arachnis hookerana
Neutral pH
Polyphenol oxidase isoforms
Temperature phase partitioning
Triton X-114
Issue Date: Nov-1999
Citation: Ho, K.-K. (1999-11). Characterization of polyphenol oxidase from aerial roots of an orchid, Aranda 'Christine 130'. Plant Physiology and Biochemistry 37 (11) : 841-848. ScholarBank@NUS Repository.
Abstract: Four isoforms of polyphenol oxidase (PPO) were demonstrated in the aerial roots of a tropical orchid, Aranda 'Christine 130'. They were extracted at neutral pH and purified to homogeneity as judged by SDS-gel electrophoresis. Purification was achieved by a combination of Triton X-114 treatment, temperature phase partitioning, gel filtration chromatography, ion-exchange separation and chromatofocusing. Two of the isoforms, designated PPO(a) and PPO(d), differed in their N-terminal sequence, tryptic peptide map and substrate affinity for (+)-catechin, but exhibited similarity in their molecular mass under denaturing conditions, pH optimum and kinetic behaviour toward 4-methyl catechol. The other two isoforms, PPO(b) and PPO(c), were identical to PPO(a) and PPO(d), respectively, in terms of their N-terminal sequence, substrate preference and pH maximum, but were different with regard to their molecular mass under denaturing conditions. These four isoforms differed in their isoelectric point.
Source Title: Plant Physiology and Biochemistry
ISSN: 09819428
DOI: 10.1016/S0981-9428(99)00114-X
Appears in Collections:Staff Publications

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