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https://doi.org/10.1016/S0981-9428(99)00114-X
Title: | Characterization of polyphenol oxidase from aerial roots of an orchid, Aranda 'Christine 130' | Authors: | Ho, K.-K. | Keywords: | Aerial roots Arachnis hookerana Chromatofocusing Neutral pH Polyphenol oxidase isoforms Temperature phase partitioning Triton X-114 |
Issue Date: | Nov-1999 | Citation: | Ho, K.-K. (1999-11). Characterization of polyphenol oxidase from aerial roots of an orchid, Aranda 'Christine 130'. Plant Physiology and Biochemistry 37 (11) : 841-848. ScholarBank@NUS Repository. https://doi.org/10.1016/S0981-9428(99)00114-X | Abstract: | Four isoforms of polyphenol oxidase (PPO) were demonstrated in the aerial roots of a tropical orchid, Aranda 'Christine 130'. They were extracted at neutral pH and purified to homogeneity as judged by SDS-gel electrophoresis. Purification was achieved by a combination of Triton X-114 treatment, temperature phase partitioning, gel filtration chromatography, ion-exchange separation and chromatofocusing. Two of the isoforms, designated PPO(a) and PPO(d), differed in their N-terminal sequence, tryptic peptide map and substrate affinity for (+)-catechin, but exhibited similarity in their molecular mass under denaturing conditions, pH optimum and kinetic behaviour toward 4-methyl catechol. The other two isoforms, PPO(b) and PPO(c), were identical to PPO(a) and PPO(d), respectively, in terms of their N-terminal sequence, substrate preference and pH maximum, but were different with regard to their molecular mass under denaturing conditions. These four isoforms differed in their isoelectric point. | Source Title: | Plant Physiology and Biochemistry | URI: | http://scholarbank.nus.edu.sg/handle/10635/100246 | ISSN: | 09819428 | DOI: | 10.1016/S0981-9428(99)00114-X |
Appears in Collections: | Staff Publications |
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