Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biochi.2006.02.010
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dc.titleCharacterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana
dc.contributor.authorHuang, W.
dc.contributor.authorLin, Z.
dc.contributor.authorSin, Y.M.
dc.contributor.authorLi, D.
dc.contributor.authorGong, Z.
dc.contributor.authorYang, D.
dc.date.accessioned2014-10-27T08:23:32Z
dc.date.available2014-10-27T08:23:32Z
dc.date.issued2006-07
dc.identifier.citationHuang, W., Lin, Z., Sin, Y.M., Li, D., Gong, Z., Yang, D. (2006-07). Characterization and expression of a cDNA encoding a tubuliform silk protein of the golden web spider Nephila antipodiana. Biochimie 88 (7) : 849-858. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biochi.2006.02.010
dc.identifier.issn03009084
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100234
dc.description.abstractSpider silks are renowned for their excellent mechanical properties. Although several spider fibroin genes, mainly from dragline and capture silks, have been identified, there are still many members in the spider fibroin gene family remain uncharacterized. In this study, a novel silk cDNA clone from the golden web spider Nephila antipodiana was isolated. It is serine rich and contains two almost identical fragments with one varied gap region and one conserved spider fibroin-like C-terminal domain. Both in situ hybridization and immunoblot analyses have shown that it is specifically expressed in the tubuliform gland. Thus, it likely encodes the silk fibroin from the tubuliform gland, which supplies the main component of the inner egg case. Unlike other silk proteins, the protein encoded by the novel cDNA in water solution exhibits the characteristic of an α-helical protein, which implies the distinct property of the egg case silk, though the fiber of tubuliform silk is mainly composed of β-sheet structure. Its sequence information facilitates elucidation of the evolutionary history of the araneoid fibroin genes. © 2006 Elsevier SAS. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biochi.2006.02.010
dc.sourceScopus
dc.subjectα-Helical structure
dc.subjectEgg case silk
dc.subjectEST
dc.subjectNephila antipodiana
dc.subjectPartial cDNA
dc.subjectTubuliform gland
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.biochi.2006.02.010
dc.description.sourcetitleBiochimie
dc.description.volume88
dc.description.issue7
dc.description.page849-858
dc.description.codenBICMB
dc.identifier.isiut000240167100012
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