Please use this identifier to cite or link to this item: https://doi.org/10.3389/fphys.2013.00362
DC FieldValue
dc.titleBranchial Na+:K+:2Cl-cotransporter 1 and Na+/K+-ATPase a-subunit in a brackish water-type ionocyte of the euryhaline freshwater white-rimmed stingray, Himantura signifer
dc.contributor.authorIp, Y.K.
dc.contributor.authorHiong, K.C.
dc.contributor.authorWong, S.Z.
dc.contributor.authorChing, B.
dc.contributor.authorChen, X.L.
dc.contributor.authorSoh, M.M.
dc.contributor.authorChng, Y.R.
dc.contributor.authorOng, J.L.
dc.contributor.authorWilson, J.M.
dc.contributor.authorChew, S.F.
dc.date.accessioned2014-10-27T08:23:07Z
dc.date.available2014-10-27T08:23:07Z
dc.date.issued2013
dc.identifier.citationIp, Y.K., Hiong, K.C., Wong, S.Z., Ching, B., Chen, X.L., Soh, M.M., Chng, Y.R., Ong, J.L., Wilson, J.M., Chew, S.F. (2013). Branchial Na+:K+:2Cl-cotransporter 1 and Na+/K+-ATPase a-subunit in a brackish water-type ionocyte of the euryhaline freshwater white-rimmed stingray, Himantura signifer. Frontiers in Physiology 4 DEC : -. ScholarBank@NUS Repository. https://doi.org/10.3389/fphys.2013.00362
dc.identifier.issn1664042X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100196
dc.description.abstractHimantura signifer is a freshwater stingray which inhabits rivers in Southeast Asia. It can survive in brackish water but not seawater. In brackish water, it becomes partially ureosmotic, but how it maintains its plasma hypoionic to the external medium is enigmatic because of the lack of a rectal gland. Here, we report for the first time the expression of Na+:K+:2Cl- cotransporter 1 (nkcc1) in the gills of freshwaterH. signifer, and its moderate up-regulation (~2-fold) in response to brackish water (salinity 20) acclimation. The absence of the Ste20-related proline-alanine-rich kinase and oxidation stress response kinase 1 interaction site from the N-terminus of H. signifer Nkcc1 suggested that it might not be effectively activated by stress kinases in response to salinity changes as in more euryhaline teleosts. The increased activity of Nkcc1 during salt excretion in brackish water would lead to an influx of Na+ into ionocytes, and the maintenance of intracellular Na+ homeostasis would need the cooperation of Na+/K+-ATPase (Nka). We demonstrated for the first time the expression of nkaa1, nkaa2 and nkaa3 in the gills of H. signifer, and the up-regulation of the mRNA expression of nkaa3 and the overall protein abundance of Nkaa in response to acclimation to brackish water. Immunofluorescence microscopy revealed the presence of a sub-type of ionocyte, co-expressing Nkcc1 and Nkaa, near the base of the secondary lamellae in the gills of H. signifer acclimated to brackish water, but this type of ionocyte was absent from the gills of fish kept in fresh water. Hence, there could be a change in the function of the gills of H. signifer from salt absorption to salt excretion during brackish water acclimation in the absence of a functioning rectal gland. © 2013 Ip, Hiong, Wong, Ching, Chen, Soh, Chng, Ong, Wilson and Chew.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.3389/fphys.2013.00362
dc.sourceScopus
dc.subjectElasmobranchs
dc.subjectGills
dc.subjectOsmoregulation
dc.subjectRectal gland
dc.subjectSalinity adaptation
dc.subjectUrea
dc.subjectUreosmotic
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.3389/fphys.2013.00362
dc.description.sourcetitleFrontiers in Physiology
dc.description.volume4 DEC
dc.description.page-
dc.identifier.isiut000346774000354
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Oct 16, 2019

WEB OF SCIENCETM
Citations

7
checked on Oct 16, 2019

Page view(s)

51
checked on Oct 12, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.