Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M112.417428
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dc.titleBiochemical characterization of the bi-lobe reveals a continuous structural network linking the bi-lobe to other single-copied organelles in Trypanosoma brucei
dc.contributor.authorGheiratmand, L.
dc.contributor.authorBrasseur, A.
dc.contributor.authorZhou, Q.
dc.contributor.authorHe, C.Y.
dc.date.accessioned2014-10-27T08:22:51Z
dc.date.available2014-10-27T08:22:51Z
dc.date.issued2013-02-01
dc.identifier.citationGheiratmand, L., Brasseur, A., Zhou, Q., He, C.Y. (2013-02-01). Biochemical characterization of the bi-lobe reveals a continuous structural network linking the bi-lobe to other single-copied organelles in Trypanosoma brucei. Journal of Biological Chemistry 288 (5) : 3489-3499. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M112.417428
dc.identifier.issn00219258
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100173
dc.description.abstractTrypanosoma brucei, a unicellular parasite, contains several single-copied organelles that duplicate and segregate in ahighly coordinated fashion during the cell cycle. In the procyclic stage, a bi-lobed structure is found adjacent to the single ER exit site and Golgi apparatus, forming both stable and dynamic association with other cytoskeletal components including the basal bodies that seed the flagellum and the flagellar pocket collar that is critical for flagellar pocket biogenesis. To further understand the bi-lobe and its association with adjacent organelles, we performed proteomic analyses on the immunoisolated bi-lobe complex. Candidate proteins were localized to the flagellar pocket, thebasal bodies,atripartite attachment complexlinking the basal bodies to the kinetoplast, and a segment of microtubule quartet linking the flagellar pocket collar and bi-lobe to the basal bodies. These results supported an extensive connection among the single-copied organelles in T. brucei, a strategy employed by the parasite for orderly organelle assembly and inheritance during the cell cycle. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M112.417428
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1074/jbc.M112.417428
dc.description.sourcetitleJournal of Biological Chemistry
dc.description.volume288
dc.description.issue5
dc.description.page3489-3499
dc.description.codenJBCHA
dc.identifier.isiut000314397900054
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