Bioactivity of the essential oil extracted from Evodia rutaecarpa Hook f. et Thomas against the grain storage insects, Sitophilus zeamais Motsch. and Tribolium castaneum (Herbst)

Abstract

The toxic, repellent and feeding deterrent activities of the essential oil extracted from Evodia rutaecarpa Hook f. et Thomas, were evaluated against Sitophilus zeamais adults and Tribolium castaneum larvae and adults. Contact toxicity assayed by topical application showed that S. zeamais adults were significantly more susceptible (LD50=0.043 μg/mg body wt) to the essential oil than T. castaneum adults (LD50=0.118 μg/mg body wt) and larvae (LD50=0.093-0.126 μg/mg body wt). However, in the fumigation assays, S. zeamais (LC50=41 μg/L air) was less susceptible to the essential oil than T. castaneum (LC50=11.7 μg/L air). When compared with larvae of various ages, T. castaneum adults were more susceptible to the fumigant toxicity of the essential oil. Also, in the treated filter paper repellency test, the essential oil was more repellent to T. castaneum than to S. zeamais. A flour disk bioassay demonstrated that the essential oil of E. rutaecarpa had a weaker feeding deterrent action against T. castaneum adults than against T. castaneum larvae and S. zeamais adults. The reduction in growth rate of T. castaneum larvae and S. zeamais adults was mainly due to a behavioural (feeding deterrent) action rather than to post-ingestive toxicity of the oil.