Please use this identifier to cite or link to this item:
|Title:||BIM is a prognostic biomarker for early prednisolone response in pediatric acute lymphoblastic leukemia||Authors:||Jiang, N.
Suang Lim, J.Y.
Yin Kham, S.K.
Juh Yeoh, A.E.
|Issue Date:||Mar-2011||Citation:||Jiang, N., Koh, G.S., Suang Lim, J.Y., Yin Kham, S.K., Ariffin, H., Chew, F.T., Juh Yeoh, A.E. (2011-03). BIM is a prognostic biomarker for early prednisolone response in pediatric acute lymphoblastic leukemia. Experimental Hematology 39 (3) : 321-329.e3. ScholarBank@NUS Repository. https://doi.org/10.1016/j.exphem.2010.11.009||Abstract:||Objective: Glucocorticoids such as prednisolone (PRED) are widely used in the treatment of pediatric acute lymphoblastic leukemia. In PRED-induced apoptosis, Bcl-2 family members play important regulatory roles. However, the exact members involved remain unknown. In this study, the roles of Bcl-2 family members in PRED-induced apoptosis and their prognostic value to day 8 PRED response are evaluated. Materials and Methods: Four clinically important acute lymphoblastic leukemia cell lines, three PRED-sensitive (697, Sup-B15, and RS4;11) and one PRED-resistant (REH) were studied. Thirty paired patient bone marrow samples were obtained at diagnosis (day 0) and after 7 days (day 8) of PRED monotherapy. Twenty-five patients had PRED good response and five PRED poor response. Differential expressions of Bcl-2 members were observed in those samples and BIM was further investigated using gene silencing technology in representative cell line Sup-B15. Results: The proapoptotic BH3-only Bcl-2 family member BIM was upregulated only in PRED-sensitive cells. Receiver operating characteristic curve analysis showed that BIM expression was highly predictive of PRED response (area under the curve = 0.81; p = 0.032) in paired patient bone marrow samples and is, most excitingly, independent of molecular subtype. Patients whose BIM protein expression levels fail to upregulate at day 8 compared to day 0 (D8/D0 ratio||Source Title:||Experimental Hematology||URI:||http://scholarbank.nus.edu.sg/handle/10635/100163||ISSN:||0301472X||DOI:||10.1016/j.exphem.2010.11.009|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 6, 2020
WEB OF SCIENCETM
checked on Jun 29, 2020
checked on Jun 27, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.