Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0063757
DC FieldValue
dc.titleAnaplastic Lymphoma Kinase Is Required for Neurogenesis in the Developing Central Nervous System of Zebrafish
dc.contributor.authorYao, S.
dc.contributor.authorCheng, M.
dc.contributor.authorZhang, Q.
dc.contributor.authorWasik, M.
dc.contributor.authorKelsh, R.
dc.contributor.authorWinkler, C.
dc.date.accessioned2014-10-27T08:21:50Z
dc.date.available2014-10-27T08:21:50Z
dc.date.issued2013-05-10
dc.identifier.citationYao, S., Cheng, M., Zhang, Q., Wasik, M., Kelsh, R., Winkler, C. (2013-05-10). Anaplastic Lymphoma Kinase Is Required for Neurogenesis in the Developing Central Nervous System of Zebrafish. PLoS ONE 8 (5) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0063757
dc.identifier.issn19326203
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100085
dc.description.abstractAnaplastic Lymphoma Kinase (ALK) was initially discovered as an oncogene in human lymphoma and other cancers, including neuroblastoma. However, little is known about the physiological function of ALK. We identified the alk ortholog in zebrafish (Danio rerio) and found that it is highly expressed in the developing central nervous system (CNS). Heat-shock inducible transgenic zebrafish lines were generated to over-express alk during early neurogenesis. Its ectopic expression resulted in activation of the MEK/ERK pathway, increased cell proliferation, and aberrant neurogenesis leading to mis-positioning of differentiated neurons. Thus, overexpressed alk is capable of promoting cell proliferation in the nervous system, similar to the situation in ALK-related cancers. Next, we used Morpholino mediated gene knock-down and a pharmacological inhibitor to interfere with expression and function of endogenous Alk. Alk inhibition did not affect neuron progenitor formation but severely compromised neuronal differentiation and neuron survival in the CNS. These data indicate that tightly controlled alk expression is critical for the balance between neural progenitor proliferation, differentiation and survival during embryonic neurogenesis. © 2013 Yao et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0063757
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pone.0063757
dc.description.sourcetitlePLoS ONE
dc.description.volume8
dc.description.issue5
dc.description.page-
dc.identifier.isiut000319055600073
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2013-anaplastic_lymphoma_kinase_required_neurogenesis-pub.pdf2.97 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

41
checked on May 12, 2022

WEB OF SCIENCETM
Citations

37
checked on May 12, 2022

Page view(s)

405
checked on May 12, 2022

Download(s)

7
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.