Please use this identifier to cite or link to this item: https://doi.org/10.1083/jcb.201210123
DC FieldValue
dc.titleAnalysis of the local organization and dynamics of cellular actin networks
dc.contributor.authorLuo, W.
dc.contributor.authorYu, C.-H.
dc.contributor.authorLieu, Z.Z.
dc.contributor.authorAllard, J.
dc.contributor.authorMogilner, A.
dc.contributor.authorSheetz, M.P.
dc.contributor.authorBershadsky, A.D.
dc.date.accessioned2014-10-27T08:21:49Z
dc.date.available2014-10-27T08:21:49Z
dc.date.issued2013
dc.identifier.citationLuo, W., Yu, C.-H., Lieu, Z.Z., Allard, J., Mogilner, A., Sheetz, M.P., Bershadsky, A.D. (2013). Analysis of the local organization and dynamics of cellular actin networks. Journal of Cell Biology 202 (7) : 1057-1073. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.201210123
dc.identifier.issn00219525
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100083
dc.description.abstractA ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the crosslinker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm. © 2013 Shigeoka et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1083/jcb.201210123
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1083/jcb.201210123
dc.description.sourcetitleJournal of Cell Biology
dc.description.volume202
dc.description.issue7
dc.description.page1057-1073
dc.description.codenJCLBA
dc.identifier.isiut000325144000008
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