Please use this identifier to cite or link to this item:
https://doi.org/10.1083/jcb.201210123
DC Field | Value | |
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dc.title | Analysis of the local organization and dynamics of cellular actin networks | |
dc.contributor.author | Luo, W. | |
dc.contributor.author | Yu, C.-H. | |
dc.contributor.author | Lieu, Z.Z. | |
dc.contributor.author | Allard, J. | |
dc.contributor.author | Mogilner, A. | |
dc.contributor.author | Sheetz, M.P. | |
dc.contributor.author | Bershadsky, A.D. | |
dc.date.accessioned | 2014-10-27T08:21:49Z | |
dc.date.available | 2014-10-27T08:21:49Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Luo, W., Yu, C.-H., Lieu, Z.Z., Allard, J., Mogilner, A., Sheetz, M.P., Bershadsky, A.D. (2013). Analysis of the local organization and dynamics of cellular actin networks. Journal of Cell Biology 202 (7) : 1057-1073. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.201210123 | |
dc.identifier.issn | 00219525 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100083 | |
dc.description.abstract | A ctin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. We studied the organization and dynamics of the actin network in nonadhesive regions of cells bridging fibronectin-coated adhesive strips. The network was formed by actin nodes associated with and linked by myosin II and containing the formin disheveled-associated activator of morphogenesis 1 (DAAM1) and the crosslinker filamin A (FlnA). After Latrunculin A (LatA) addition, actin nodes appeared to be more prominent and demonstrated drift-diffusion motion. Superresolution microscopy revealed that, in untreated cells, DAAM1 formed patches with a similar spatial arrangement to the actin nodes. Node movement (diffusion coefficient and velocity) in LatA-treated cells was dependent on the level and activity of myosin IIA, DAAM1, and FlnA. Based on our results, we developed a computational model of the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. We suggest that such networks are critical for connecting distant parts of the cell to maintain the mechanical coherence of the cytoplasm. © 2013 Shigeoka et al. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1083/jcb.201210123 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1083/jcb.201210123 | |
dc.description.sourcetitle | Journal of Cell Biology | |
dc.description.volume | 202 | |
dc.description.issue | 7 | |
dc.description.page | 1057-1073 | |
dc.description.coden | JCLBA | |
dc.identifier.isiut | 000325144000008 | |
Appears in Collections: | Staff Publications |
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