Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.virol.2013.11.021
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dc.titleAn infectious RNA with a hepta-adenosine stretch responsible for programmed -1 ribosomal frameshift derived from a full-length cDNA clone of Hibiscus latent Singapore virus
dc.contributor.authorNiu, S.
dc.contributor.authorCao, S.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-10-27T08:21:39Z
dc.date.available2014-10-27T08:21:39Z
dc.date.issued2014-01-20
dc.identifier.citationNiu, S., Cao, S., Wong, S.-M. (2014-01-20). An infectious RNA with a hepta-adenosine stretch responsible for programmed -1 ribosomal frameshift derived from a full-length cDNA clone of Hibiscus latent Singapore virus. Virology 449 : 229-234. ScholarBank@NUS Repository. https://doi.org/10.1016/j.virol.2013.11.021
dc.identifier.issn00426822
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100070
dc.description.abstractHibiscus latent Singapore virus (HLSV) is a member of Tobamovirus and its full-length cDNA clones were constructed. The in vitro transcripts from two HLSV full-length cDNA clones, which contain a hepta-adenosine stretch (pHLSV-7A) and an octo-adenosine stretch (pHLSV-8A), are both infectious. The replication level of HLSV-7A in Nicotiana benthamiana protoplasts was 5-fold lower, as compared to that of HLSV-8A. The replicase proteins of HLSV-7A were produced through programmed -1 ribosomal frameshift (-1 PRF) and the 7A stretch was a slippery sequence for -1 PRF. Mutations to the downstream pseudoknot of 7A stretch showed that the pseudoknot was not required for the frameshift in vitro. The stretch was found to be extended to 8A after subsequent replication cycles in vivo. It is envisaged that HLSV employs the monotonous runs of A and -1 PRF to convert its 7A to 8A to reach higher replication for its survival in plants. © 2013 Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.virol.2013.11.021
dc.sourceScopus
dc.subject-1 PRF
dc.subjectBiologically active cDNA clone
dc.subjectDownstream pseudoknot
dc.subjectHepta-adenosine stretch
dc.subjectTobamovirus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.virol.2013.11.021
dc.description.sourcetitleVirology
dc.description.volume449
dc.description.page229-234
dc.description.codenVIRLA
dc.identifier.isiut000330094100026
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