Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.febslet.2010.11.060
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dc.titleAn adjacent arginine, and the phosphorylated tyrosine in the c-Met receptor target sequence, dictates the orientation of c-Cbl binding
dc.contributor.authorSun, Q.
dc.contributor.authorNg, C.
dc.contributor.authorGuy, G.R.
dc.contributor.authorSivaraman, J.
dc.date.accessioned2014-10-27T08:21:27Z
dc.date.available2014-10-27T08:21:27Z
dc.date.issued2011-01-21
dc.identifier.citationSun, Q., Ng, C., Guy, G.R., Sivaraman, J. (2011-01-21). An adjacent arginine, and the phosphorylated tyrosine in the c-Met receptor target sequence, dictates the orientation of c-Cbl binding. FEBS Letters 585 (2) : 281-285. ScholarBank@NUS Repository. https://doi.org/10.1016/j.febslet.2010.11.060
dc.identifier.issn00145793
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100050
dc.description.abstractPreviously, we have demonstrated that the tyrosine phosphorylated hepatocyte growth factor receptor (Met) binds to the c-Cbl phosphotyrosine- recognition, tyrosine kinase binding (TKB) domain in a reverse orientation compared to other c-Cbl binding partners. A Met peptide with the DpYR motif changed to RpYD (MetRD) retains a similar TKB binding affinity as the native Met peptide. However, the TKB: MetRD complex crystal structure reveals a complete reversal of the binding orientation. Collated data indicates that both binding and orientation is dictated by the phosphorylated tyrosine and an adjacent arginine forming intra-peptide hydrogen bonds and aligning unidirectionally with complementary charges in the phosphotyrosine binding pocket of c-Cbl. Structured summary: c-Cbl and MetRD bind: shown by x-ray crystallography (view interaction) c-Cbl and MetRD bind: shown by mass spectrometry studies of complexes (view interaction) c-Cbl bind to Met: shown by surface plasmon resonance (view interactions 1,2). © 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.febslet.2010.11.060
dc.sourceScopus
dc.subjectc-Casitas B-lineage lymphoma tyrosine kinase binding domain
dc.subjectCrystal structure
dc.subjectHepatocyte growth factor receptor
dc.subjectReverse binding
dc.subjectSurface plasmon resonance
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.febslet.2010.11.060
dc.description.sourcetitleFEBS Letters
dc.description.volume585
dc.description.issue2
dc.description.page281-285
dc.description.codenFEBLA
dc.identifier.isiut000286180300003
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