Please use this identifier to cite or link to this item: https://doi.org/10.1111/pcmr.12183
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dc.titleAlternative transcription generates multiple Mitf isoforms with different expression patterns and activities in medaka
dc.contributor.authorLi, M.
dc.contributor.authorZhu, F.
dc.contributor.authorHong, N.
dc.contributor.authorZhang, L.
dc.contributor.authorHong, Y.
dc.date.accessioned2014-10-27T08:21:19Z
dc.date.available2014-10-27T08:21:19Z
dc.date.issued2014-01
dc.identifier.citationLi, M., Zhu, F., Hong, N., Zhang, L., Hong, Y. (2014-01). Alternative transcription generates multiple Mitf isoforms with different expression patterns and activities in medaka. Pigment Cell and Melanoma Research 27 (1) : 48-58. ScholarBank@NUS Repository. https://doi.org/10.1111/pcmr.12183
dc.identifier.issn17551471
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100039
dc.description.abstractMicrophthalmia-associated transcription factor (Mitf) is best known for distinct functions in multiple cell lineages including melanocytes, mast cells, and osteoclasts. In mammals, mitf produces multiple Mitf isoforms by alternative transcription and splicing. The fish medaka has two mitf genes, mitf1 and mitf2. Here, we report differential expression and activities of medaka Mitf isoforms. Molecular cloning identified four mitf1 variants encoding isoforms Mitf1A, MitfB, MitfH, and MitfM, and only one mitf2RNA encoding Mitf2M, which exhibited differential expression. Mitf1 isoforms and Mitf2M differed dramatically in activating the dazl and tyrosinase promoters DAZ and TYR. Interestingly, Mitf1ΔN, an N-terminus-less Mitf1 mutant form, retained activity to activate TYR but not DAZ. Importantly, Mitf1B was also sufficient for inducing melanocyte differentiation and endogenous tyrosinase RNA expression in medaka embryonic stem cells. Intriguingly, Mitf1 isoforms possessed considerable differences in inducing the expression of multiple cell lineage marker genes. Therefore, alternative mitf transcription is a conserved mechanism from fish to mammals, and medaka Mitf1 isoforms show differences in expression and activity. We conclude that differential expression of isoforms contributes to multiple distinct functions of Mitf in vertebrates. © 2013 John Wiley & Sons A/S.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/pcmr.12183
dc.sourceScopus
dc.subjectAlternative transcription
dc.subjectIsoform
dc.subjectMelanocyte
dc.subjectMitf
dc.subjectPigmentation
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentPHARMACY
dc.description.doi10.1111/pcmr.12183
dc.description.sourcetitlePigment Cell and Melanoma Research
dc.description.volume27
dc.description.issue1
dc.description.page48-58
dc.identifier.isiut000328631100011
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