Please use this identifier to cite or link to this item: https://doi.org/10.1007/s002990100334
Title: Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1
Authors: Yu, H. 
Yang, S.H.
Goh, C.J. 
Keywords: Agrobacterium tumefaciens
DOH1
Orchid
Plant architecture
Transformation
Issue Date: 2001
Citation: Yu, H.,Yang, S.H.,Goh, C.J. (2001). Agrobacterium-mediated transformation of a Dendrobium orchid with the class 1 knox gene DOH1. Plant Cell Reports 20 (4) : 301-305. ScholarBank@NUS Repository. https://doi.org/10.1007/s002990100334
Abstract: Transgenic orchid (Dendrobium Madame Thong-In) plants were regenerated by inoculating thin-section explants from protocorm-like bodies (PLBs) with Agrobacterium tumefaciens strain LBA4404 harboring a binary vector that carried the orchid DOH1 antisense gene. The transformation was performed through two consecutive stages of cocultivation, with the first stage occurring on antibiotic-free medium for 3 days and the subsequent stage on medium containing 50 mg/l carbenicillin for 3-4 weeks. Proliferated PLBs were repeatedly selected for kanamycin resistance. The presence and expression of the transgene were assessed by molecular analyses. Expression of the DOH1 antisense gene caused abnormal multiple shoot development, indicating a role for DOH1 in the basic plant architecture in orchid.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/100026
ISSN: 07217714
DOI: 10.1007/s002990100334
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