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Expanding the scope of site-specific protein biotinylation strategies using small molecules

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Alternative Title
Abstract
We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods. © 2004 Elsevier Ltd. All rights reserved.
Keywords
Biotinylation, Cell-free protein expression, Microarray, Protein, Puromycin
Source Title
Bioorganic and Medicinal Chemistry Letters
Publisher
Series/Report No.
Organizational Units
Organizational Unit
CHEMISTRY
dept
Organizational Unit
BIOLOGICAL SCIENCES
dept
Rights
Date
2004-12-06
DOI
10.1016/j.bmcl.2004.09.056
Type
Article
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