A small peptide derived from Flt-1 (VEGFR-1) functions as an angiogenic inhibitor
Tan, D.C.W. ; Kini, R.Manjunatha ; Jois, S.D.S. ; Lim, D.K.F. ; Xin, L. ; Ge, R.
Tan, D.C.W.
Lim, D.K.F.
Xin, L.
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Alternative Title
Abstract
Vascular endothelial growth factor (VEGF) is an angiogenic stimulator which functions through two endothelial specific tyrosine kinase receptors, Flt-1 and Flk-1. In this work, we show that an 11-amino acid peptide derived from the second immunoglobulin-like domain of Flt-1 functions as an angiogenic inhibitor in chick chorioallantoic membrane and inhibited VEGF-induced vascular permeability in Miles' assay without binding to VEGF directly. Circular dichroism and nuclear magnetic resonance analyses indicate that this peptide forms a stable extended structure in solution, presumably β-sheet structure and is most likely existing as a dimer. Our results suggest that this small peptide functions as an angiogenic inhibitor by inhibiting VEGF function through a non-VEGF binding mechanism. © 2001 Federation of European Biochemical Societies.
Keywords
Angiogenesis, Chick chorioallantoic membrane, Flt-1(VEGFR-1), Miles' assay, Vascular endothelial growth factor (vascular permeability factor)
Source Title
FEBS Letters
Publisher
Series/Report No.
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Date
2001-04-13
DOI
10.1016/S0014-5793(01)02314-6
Type
Article