Site-specific immobilization of proteins in a microarray using intein-mediated protein splicing
Girish, A. Sun, H. Yeo, D.S.Y. Chen, G.Y.J. Chua, T.-K.
Girish, A.
Sun, H.
Yeo, D.S.Y.
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Alternative Title
Abstract
One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. Herein, we report a bacterium-based, intein-mediated strategy to generate N-terminal cysteine-containing proteins which are then chemoselectively immobilized to a thioester-functionalized glass slide to generate the corresponding protein microarray. We also showed preliminary data of the strategy in a yeast host system. © 2005 Elsevier Ltd. All rights reserved.
Keywords
Intein, N-terminal cysteine-containing protein, Protein array, Proteomics
Source Title
Bioorganic and Medicinal Chemistry Letters
Publisher
Series/Report No.
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Date
2005-05-16
DOI
10.1016/j.bmcl.2005.03.079
Type
Article