A fast assay for developing serum free media for cord blood haematopoietic cells
Oh, S.K.W. Ng, T. See, L.H.
Ng, T.
See, L.H.
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Abstract
One of the bottlenecks in haematopoietic cell expansion is the lack of a defined serum free media. We describe a fast method of monitoring live haematopoietic cell numbers, using fluorescein diacetate, to facilitate the development of such a media. Several carbohydrates (fructose at 500μg/ml and pyruvate at 300μg/ml) and lipids (cholesterol and lecithin, both at 10μg/ml) were tested and could replace serum in cord blood haematopoietic cell cultures. © 1995 Chapman & Hall.
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Source Title
Biotechnology Techniques
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Date
1995-08
DOI
10.1007/BF00152447
Type
Article