Bioreduction of trivalent aurum to nano-crystalline gold particles by active and inactive cells and cell-free extract of Aspergillus oryzae var. viridis
Binupriya, A.R. Sathishkumar, M. Vijayaraghavan, K. Yun, S.-I.
Binupriya, A.R.
Yun, S.-I.
Citations
Altmetric:
Alternative Title
Abstract
Bioreduction efficacy of both active (AB) and inactive (IB) cells/biomass of Aspergillus oryzae var. viridis and their respective cell-free extracts (ACE and ICE) to convert trivalent aurum to gold nanoparticles were tested in the present study. Strong plasmon resonance of gold nanoparticles was observed between 540 and 560. nm in the samples obtained from AB, IB, ACE and ICE. Transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) were performed to examine the formation of gold nanoparticles. Comparing all four forms of A. oryzae var. viridis, ICE showed high gold nanoparticle productivity. The nanoparticles formed were quite uniform in shape and ranged in size from 10 to 60. nm. In addition some triangle, pentagon and hexagon-shaped nanoplates with size range of 30-400. nm were also synthesized especially at lower pH. Organics from the inactive cells are believed to be responsible for reduction of trivalent aurum to nano-sized gold particles. Organic content of the ICE was found to be double the amount of ACE. High productivity of gold nanoparticles by metabolic-independent process opens up an interesting area of nanoparticle synthesis using waste fungal biomass from industries. © 2009 Elsevier B.V.
Keywords
Active and inactive biomass, Aspergillus oryzae var. viridis, Bioreduction, Cell-free extract, Gold nanoparticles
Source Title
Journal of Hazardous Materials
Publisher
Series/Report No.
Collections
Rights
Date
2010-05
DOI
10.1016/j.jhazmat.2009.12.066
Type
Article