Deep sequencing in infectious diseases: Immune and pathogen repertoires for the improvement of patient outcomes
Burkholder, W.F Newell, E.W Poidinger, M Chen, S Fink, K
Burkholder, W.F
Newell, E.W
Fink, K
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Alternative Title
Abstract
The inaugural workshop "Deep Sequencing in Infectious Diseases: Immune and Pathogen Repertoires for the Improvement of Patient Outcomes" was held in Singapore on 13-14 October 2016. The aim of the workshop was to discuss the latest trends in using high-throughput sequencing, bioinformatics, and allied technologies to analyze immune and pathogen repertoires and their interplay within the host, bringing together key international players in the field and Singapore-based researchers and clinician-scientists. The focus was in particular on the application of these technologies for the improvement of patient diagnosis, prognosis and treatment, and for other broad public health outcomes. The presentations by scientists and clinicians showed the potential of deep sequencing technology to capture the coevolution of adaptive immunity and pathogens. For clinical applications, some key challenges remain, such as the long turnaround time and relatively high cost of deep sequencing for pathogen identification and characterization and the lack of international standardization in immune repertoire analysis. @ 2017 Burkholder, Newell, Poidinger, Chen and Fink.
Keywords
B lymphocyte receptor, T lymphocyte receptor, adaptive immunity, Article, atopic dermatitis, bacterial colonization, bioinformatics, deep sequencing, disease transmission, DNA sequence, droplet digital polymerase chain reaction, flow cytometry, gene library, Hepatitis B virus, high throughput sequencing, immunological memory, infection, methicillin resistant Staphylococcus aureus, next generation sequencing, polymerase chain reaction, polymerase chain reaction cloning, RNA sequence, scientist, single cell sequencing, treatment outcome, turnaround time, vaccination, VDJ recombination
Source Title
Frontiers in Immunology
Publisher
Series/Report No.
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Rights
Attribution 4.0 International
Date
2017
DOI
10.3389/fimmu.2017.00593
Type
Article