Physicochemical and blood compatibility characterization of polypyrrole surface functionalized with heparin
Li, Y. Neoh, K.G. Cen, L. Kang, E.-T.
Li, Y.
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Alternative Title
Abstract
A surface modification technique was developed for the covalent immobilization of heparin onto electrically conductive polypyrrole (PPY) film. The PPY film was first graft copolymerized with poly(ethylene glycol) methacrylate (PEGMA) and then activated with cyanuric chloride (CC). Heparin was then immobilized onto the film through the reaction between the chloride groups of CC and the amine and/or hydroxyl groups of heparin. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified film after each stage. The biocompatibility of the surface-modified PPY was evaluated using plasma recalcification time (PRT) and platelet adhesion. After surface modification, the film had improved wettability while retaining significant electrical conductivity. With immobilized heparin, platelet adhesion and platelet activation on PPY film was significantly suppressed, and the PRT was significantly prolonged. Electrical stimulation also plays a positive role in decreasing platelet adhesion and increasing PRT on pristine and surface-modified PPY films. © 2003 Wiley Periodicals, Inc.
Keywords
Electrical stimulation, Heparin, Plasma recalcification time, Platelet adhesion, Polypyrrole, Surface modification
Source Title
Biotechnology and Bioengineering
Publisher
Series/Report No.
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Date
2003-11-05
DOI
10.1002/bit.10757
Type
Article