An AIEgen-Peptide Conjugate as a Phototheranostic Agent for Phagosome-Entrapped Bacteria
Qi, Guobin ; Hu, Fang ; Kenry ; Shi, Leilei ; Wu, Min ; Liu, Bin
Qi, Guobin
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Abstract
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim The detection and elimination of intracellular bacteria remain a major challenge. In this work, we report an aggregation-induced emission (AIE) bioprobe that can detect bacterial infection and kill bacteria surviving inside macrophages through a dynamic process, notably specific molecular tailoring of the probe by caspase-1 activation in infected macrophages and accumulation of the residue on phagosomes containing bacteria, leading to light-up fluorescent signals. Moreover, the AIEgen can serve as a photosensitizer for generation of reactive oxygen species (ROS); and the average ROS indicator fluorescent signal intensity per unit area in the bacterial phagosomes is approximately 2.7-fold higher than that in the cytoplasm. This, in turn, induces bacteria killing with high efficiency and minimal cytotoxicity towards macrophages. We envision that this specific light-up bioprobe may provide a new approach for selective and sensitive detection and eradication of intracellular bacterial infections.
Keywords
Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, aggregation-induced emission, antimicrobial agents, fluorescence imaging, infection, photodynamic therapy, FLUORESCENT-PROBES, CASPASE-1, INFLAMMASOME, RECOGNITION, INFECTION, ENZYME
Source Title
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Publisher
WILEY-V C H VERLAG GMBH
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Date
2019-11-04
DOI
10.1002/anie.201906099
Type
Article