Ensuring oxygenation of carrot cell cultures in a Couette viscometer during investigation of shear effects
Wong, V. Williams, D. Colby, C.B.
Williams, D.
Colby, C.B.
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Abstract
Mathematical simulation and experimental measurement of dissolved O2 were performed for extended (up to 8 h) shear testing of Daucus carota (carrot) cell cultures in a conventional Couette viscometer (0.625 mm annulus). The results suggest O2 depletion below critical levels for cell growth may occur. A novel design modification incorporating an O2-permeable silicone-layer spun cast on a porous ceramic bowl was devised. It significantly improved oxygenation of the cell cultures, keeping dissolved O2 near saturation.
Keywords
Couette viscometer, Mathematical simulation, Oxygen depletion, Plant cell culture, Shear sensitivity
Source Title
Biotechnology Letters
Publisher
Series/Report No.
Collections
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Date
2001
DOI
10.1023/A:1012766116568
Type
Article