High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
Bevilacqua, C Gomez, JM Fiuza, UM Chan, CJ Wang, L Hambura, S Eguren, M Ellenberg, J Diz-Muñoz, A Leptin, M
Bevilacqua, C
Gomez, JM
Fiuza, UM
Wang, L
Hambura, S
Eguren, M
Ellenberg, J
Diz-Muñoz, A
Leptin, M
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Alternative Title
Abstract
Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.
Keywords
Animals, Mice, Microscopy, Embryonic Development, Drosophila, Embryo, Nonmammalian, Imaging, Three-Dimensional
Source Title
Nature Methods
Publisher
Springer Science and Business Media LLC
Series/Report No.
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Date
2023-03-30
DOI
10.1038/s41592-023-01822-1
Type
Article