Gene expression profiling to define host response to baculoviral transduction in the brain
Boulaire, J. Zhao, Y. Wang, S.
Boulaire, J.
Zhao, Y.
Citations
Altmetric:
Alternative Title
Abstract
Recombinant baculoviral vectors efficiently transduce several types of cells in the brain. To characterize host responses to virus challenge, thus verifying the suitability of using baculovirus for the development of gene therapy strategies in the central nervous system, we used cDNA microarray technology to examine in vitro and in vivo global cellular gene expression profiles in the rat brain, cultured human astrocytes and human neuronal cells after viral transduction. We demonstrated that the transduction induced host antiviral responses as a major reaction in all three types of samples profiled. The related genes were mainly those associated with innate immunity, including several of the genes involved in Toll-like receptor signaling pathway and cytokine-cytokine receptor interaction. These findings should be useful in understanding the molecular basis for neural cell response to baculoviral transduction and in guiding rational therapeutic applications of baculoviral vectors in the central nervous systems. © 2009 International Society for Neurochemistry.
Keywords
Astrocytes, Baculovirus, Brain, DNA microarray, Neurons, Transduction
Source Title
Journal of Neurochemistry
Publisher
Series/Report No.
Collections
Rights
Date
2009-06
DOI
10.1111/j.1471-4159.2009.06015.x
Type
Article