Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis
Yao, Y.J. Loh, K.C. Chung, M.C.M. Li, S.F.Y.
Yao, Y.J.
Chung, M.C.M.
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Alternative Title
Abstract
Capillary electrophoresis was used for the analysis of recombinnt tumor necrosis factor beta (rhTNF-β). Reproducible separation of the protein could be achieved in Polybrene-coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybrene-coated capillaries, other commercially available column and column-coating reagents were also examined for the separation of rhTNF-β. Capillary electrophoresis was found to be a potentially useful method for product analysis and process monitoring in recombinant-DNA technology.
Keywords
capillary electrophoresis, recombinant-DNA technology, tumor necrosis factor
Source Title
Electrophoresis
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Series/Report No.
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Date
1995
DOI
Type
Conference Paper