A comparative genomics and reductive dehalogenase gene transcription study of two chloroethene-respiring bacteria, Dehalococcoides mccartyi strains MB and 11a
Low, A Shen, Z Cheng, D Rogers, M.J Lee, P.K.H He, J
Shen, Z
Lee, P.K.H
Citations
Altmetric:
Alternative Title
Abstract
Genomes of two trichloroethene (TCE)-respiring Dehalococcoides (Dhc) mccartyi, strains MB and 11a, were sequenced to identify reductive dehalogenases (RDase) responsible for oraganohalide respiration. Transcription analyses were conducted to verify the roles of RDase subunit A genes (rdhA) in chloroethene respiration. Some interesting features of the strain MB draft genome include a large genome size, two CRISPR-cas type I systems, and 38 rdhA genes. Strain 11a has a stream-lined genome with 11 rdhA genes, of which nine are distinct. Quantitative real-time PCR transcription analysis of RDase gene transcripts showed that a single RDase gene, designated mbrA, was up-regulated upon exposure to TCE and no other RDase genes were considerably expressed in strain MB. A single RDase gene, designated vcrA, was up-regulated upon exposure to TCE and expressed at a steady level until all chloroethenes were completely dechlorinated to ethene at 147 h in strain 11a. Overall, this study reports the genomes of two distinct Dhc strains; both contain numerous uncharacterized RDase genes, but in each strain only one such gene was expressed highly during organohalide respiration.
Keywords
bacterial protein, trichloroethylene, bacterial gene, bacterial genome, bacterium, comparative study, genetic transcription, genetics, genomics, halogenation, metabolism, procedures, upregulation, Bacteria, Bacterial Proteins, Genes, Bacterial, Genome, Bacterial, Genomics, Halogenation, Transcription, Genetic, Trichloroethylene, Up-Regulation
Source Title
Scientific Reports
Publisher
Nature Publishing Group
Series/Report No.
Collections
Rights
Date
2015
DOI
10.1038/srep15204
Type
Article