Field-based flow cytometry for ex vivo characterization of plasmodium vivax and P. falciparum antimalarial sensitivity
Russell B. Malleret B. Suwanarusk R. Anthony C. Kanlaya S. Lau Y.L. Woodrow C.J. Nosten F. Renia L.
Suwanarusk R.
Anthony C.
Kanlaya S.
Lau Y.L.
Woodrow C.J.
Nosten F.
Citations
Altmetric:
Alternative Title
Abstract
Ex vivo antimalarial sensitivity testing in human malaria parasites has largely depended on microscopic determination of schizont maturation. While this microscopic method is sensitive, it suffers from poor precision and is laborious. The recent development of portable, low-cost cytometers has allowed us to develop and validate a simple, field-optimized protocol using SYBR green and dihydroethidium for the accurate and objective determination of antimalarial drug sensitivity in freshly isolated Plasmodium vivax and Plasmodium falciparum. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Keywords
artesunate, chloroquine, fluorescent dye, hydroethidine, antimalarial drug susceptibility, article, clinical protocol, controlled study, ex vivo study, flow cytometry, Giemsa stain, human, IC 50, major clinical study, microscopy, parasitemia, Plasmodium falciparum, Plasmodium vivax, priority journal, Antimalarials, Flow Cytometry, Plasmodium falciparum, Plasmodium vivax
Source Title
Antimicrobial Agents and Chemotherapy
Publisher
Series/Report No.
Collections
Rights
Date
2013
DOI
10.1128/AAC.00682-13
Type
Article