A novel subset of NK cells expressing high levels of inhibitory Fc?RIIB modulating antibody-dependent function
Dutertre, C.-A Bonnin-Gélizé, E Pulford, K Bourel, D Fridman, W.-H Teillaud, J.-L
Bonnin-Gélizé, E
Pulford, K
Bourel, D
Fridman, W.-H
Teillaud, J.-L
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Alternative Title
Abstract
NK cells can kill antibody-coated target cells following engagement of Fc?RIIIA, the major activating Fc?R expressed by these cells. The presence of Fc?RIIC (CD32C) has also been reported, but its contribution to the Fc?R-dependent effector functions of NK cells remains debated. We demonstrate here that inhibitory Fc?RIIB is also expressed by a small subset of CD56+/NKp46+ NK cells and can efficiently down-modulate their Fc?R-dependent effector function. Immunofluorescence analyses of NK cells from 52 healthy donors showed the presence of CD56 bright/Fc?RII-(5.2%±3.4), CD56dim/ Fc?RIIlo/- (94.1%±3.4), and CD56dim/ Fc?RIIbright (0.64%±0.72) cells. QRT-PCR and protein analyses performed on isolated Fc?RIIbright NK cells indicated that Fc?RIIB is strongly expressed by these cells but not by Fc?RIIlo/- cells. In addition, Fc?RIIbright cells showed a weaker antibody-dependent degranulation when incubated with IgG-coated target cells compared with Fc?RIIlo/- NK cells, although a strong Fc?RIIIA expression was detected in both cells. Furthermore, the addition of anti-Fc?RII Fab paralleled a higher degranulation of Fc?RIIbright NK cells, indicating a direct role for Fc?RIIB in this down-modulating effect. Thus, it is proposed that Fc?RIIBbright NK cells represent a new NK cell compartment able to down-modulate NK cell functions triggered by the engagement of activating Fc?R. © Society for Leukocyte Biology.
Keywords
antibody, Fc receptor IIb, immunoglobulin G, antigen expression, article, cell function, controlled study, human, human cell, immunofluorescence, natural killer cell, priority journal, reverse transcription polymerase chain reaction, Antigens, CD, Blotting, Western, Cell Culture Techniques, Flow Cytometry, Fluorescent Antibody Technique, Humans, Immunoglobulin G, Immunoprecipitation, Killer Cells, Natural, Lymphocyte Subsets, Phenotype, Receptors, IgG
Source Title
Journal of Leukocyte Biology
Publisher
Series/Report No.
Collections
Rights
Attribution 4.0 International
Date
2008
DOI
10.1189/jlb.0608343
Type
Article