Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis
Chow, G. Nietfeld, J.J. Knudson, C.B. Knudson, W.
Nietfeld, J.J.
Knudson, C.B.
Knudson, W.
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Abstract
Objective. To better define critical functions of the hyaluronan receptor CD44 in cartilage. Methods. Articular chondrocytes and cartilage tissue slices were treated with CD44 sequence-specific antisense phosphorothioate oligonucleotides. CD44 expression was probed by immunofluorescence microscopy, enzyme-linked immunosorbent assay, and Western blotting. Results. Antisense oligonucleotides demonstrated a dose- and time- dependent inhibition of CD44 protein expression; negative controls showed no effect. Similar to osteoarthritic cartilage, antisense-treated cartilage slices displayed a near-total loss of stainable proteoglycan-rich matrix. Conclusion. CD44 expression is needed for maintenance of cartilage homeostasis.
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Source Title
Arthritis and Rheumatism
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Series/Report No.
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Date
1998-08
DOI
10.1002/1529-0131(199808)41:83.0.CO;2-Z
Type
Article