RAJ KUMAR DAS
Email Address
chmrkd@nus.edu.sg
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Publication Development of background-free tame fluorescent probes for intracellular live cell imaging(Nature Publishing Group, 2016) Alamudi, S.H; Satapathy, R; Kim, J; Su, D; Ren, H; Das, R; Hu, L; Alvarado-Martínez, E; Lee, J.Y; Hoppmann, C; Penã-Cabrera, E; Ha, H.-H; Park, H.-S; Wang, L; Chang, Y.-T; LIFE SCIENCES INSTITUTE; CHEMISTRYFluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy to achieve in-depth understanding of the biomolecule's roles and functions. Besides being nontoxic and specific, desirable labelling probes should be highly cell permeable without nonspecific interactions with other cellular components to warrant high signal-to-noise ratio. While it is critical, rational design for such probes is tricky. Here we report the first predictive model for cell permeable background-free probe development through optimized lipophilicity, water solubility and charged van der Waals surface area. The model was developed by utilizing high-throughput screening in combination with cheminformatics. We demonstrate its reliability by developing CO-1 and AzG-1, a cyclooctyne-and azide-containing BODIPY probe, respectively, which specifically label intracellular target organelles and engineered proteins with minimum background. The results provide an efficient strategy for development of background-free probes, referred to as 'tame' probes, and novel tools for live cell intracellular imaging.Publication In vivo detection of macrophage recruitment in hind-limb ischemia using a targeted near-infrared fluorophore(Public Library of Science, 2014) Yoo J.S.; Das R.K.; Jow Z.Y.; Chang Y.-T.; CHEMISTRY