Chow Jia Xin

Email Address
gmscjx@nus.edu.sg


Organizational Units
OrgUnit Logo
Organizational Unit
DUKE-NUS MEDICAL SCHOOL
faculty
OrgUnit Logo
Organizational Unit

Filters

20191
Reset filters

Settings

Citations

Publication Search Results

Now showing 1 - 1 of 1
  • No Thumbnail Available
    Publication
    Spatiotemporal Differences in Presentation of CD8 T Cell Epitopes during Hepatitis B Virus Infection
    (AMERICAN SOCIETY FOR MICROBIOLOGY, 2019-02-01) Khakpoor, Atefeh; Ni, Yi; Chen, Antony; Ho, Zi Zong; Oei, Vincent; Yang, Ninghan; Giri, Reshmi; Chow, Jia Xin; Tan, Anthony T; Kennedy, Patrick T; Maini, Mala; Urban, Stephan; Bertoletti, Antonio; Dr Atefeh Khakpoor; DUKE-NUS MEDICAL SCHOOL
    Copyright © 2019 Khakpoor et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Distinct populations of hepatocytes infected with hepatitis B virus (HBV) or only harboring HBV DNA integrations coexist within an HBV chronically infected liver. These hepatocytes express HBV antigens at different levels and with different intracellular localizations, but it is not known whether this heterogeneity of viral antigen expression could result in an uneven hepatic presentation of distinct HBV epitopes/HLA class I complexes triggering different levels of activation of HBV-specific CD8 T cells. Using antibodies specific to two distinct HLA-A*02:01/HBV epitope complexes of HBV nucleocapsid and envelope proteins, we mapped their topological distributions in liver biopsy specimens of two anti-hepatitis B e antigen-positive (HBe) chronic HBV (CHB) patients. We demonstrated that the core and envelope CD8 T cell epitopes were not uniformly distributed in the liver parenchyma but preferentially located in distinct and sometimes mutually exclusive hepatic zones. The efficiency of HBV epitope presentation was then tested in vitro utilizing HLA-A*02:01/HBV epitope-specific antibodies and the corresponding CD8 T cells in primary human hepatocyte and hepatoma cell lines either infected with HBV or harboring HBV DNA integration. We confirmed the existence of a marked variability in the efficiency of HLA class I/HBV epitope presentation among the different targets that was influenced by the presence of gamma interferon (IFN-) and availability of newly translated viral antigens. In conclusion, HBV antigen presentation can be heterogeneous within an HBV-infected liver. As a consequence, CD8 T cells of different HBV specificities might have different antiviral efficacies.