Li Ying Nicole Lee
Email Address
dbsllyn@nus.edu.sg
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Publication The microbiome of the seagrass, Halophila ovalis: community structuring from plant parts to regional scales(2021-07-27) Bertrand Chengxiang Yan; Golam Rabbani; Nicole Li Ying Lee; Jillian Lean Sim Ooi; Jen Nie Lee; Huang Danwei; BENJAMIN JOHN WAINWRIGHT; YALE-NUS COLLEGE; BIOLOGICAL SCIENCESPublication Biogeographic structure of fungal communities in seagrass Halophilia ovalis across the Malay Peninsula(2021-08-30) ZHENG BIN, RANDOLPH QUEK; Geoffrey Zahn; LEE LI YING NICOLE; Jillian Lean Sim Ooi; Jen Nie Lee; HUANG DANWEI; BENJAMIN JOHN WAINWRIGHT; YALE-NUS COLLEGE; BIOLOGICAL SCIENCESPublication Mangrove-associated fungal communities are differentiated by geographic location and host structure(Frontiers Media S.A., 2019) Lee, N.L.Y.; Huang, D.; Quek, Z.B.R.; Lee, J.N.; Wainwright, B.J.; BIOLOGICAL SCIENCESMarine fungi on the whole remain understudied, especially in the highly diverse Southeast Asian region. We investigated the fungal communities associated with the mangrove tree Avicennia alba throughout Singapore and Peninsular Malaysia. At each sampling location, we examined ten individual trees, collecting leaves, fruits, pneumatophores, and an adjacent sediment sample from each plant. Amplicon sequencing of the fungal internal transcribed spacer 1 and subsequent analyses reveal significant differences in fungal communities collected from different locations and host structures. Mantel tests and multiple regression on distance matrices show a significant pattern of distance decay with samples collected close to one another having more similar fungal communities than those farther away. Submergence appears to drive part of the variation as host structures that are never submerged (leaves and fruits) have more similar fungal communities relative to those that are covered by water during high tide (pneumatophores and sediment). We suggest that fungi of terrestrial origins dominate structures that are not inundated by tidal regimes, while marine fungi dominate mangrove parts and sediments that are submerged by the incoming tide. Given the critical functions fungi play in all plants, and the important role they can have in determining the success of restoration schemes, we advocate that fungal community composition should be a key consideration in any mangrove restoration or rehabilitation project. © 2019 Lee, Huang, Quek, Lee and Wainwright.Publication Spatial and Structural Factors Shape Seagrass-Associated Bacterial Communities in Singapore and Peninsular Malaysia(Frontiers Media S.A., 2021-05-31) Rabbani, Golam; Yan, Bertrand Chengxiang; Lee, Nicole Li Ying; Ooi, Jillian Lean Sim; Lee, Jen Nie; Huang, Danwei; Wainwright, Benjamin J.; DEAN'S OFFICE (YALE-NUS COLLEGE); BIOLOGICAL SCIENCESPlant-microbe relationships play critical roles in the functioning and health of terrestrial plants, but little is known about this relationship in marine angiosperms such as seagrasses. Here, we investigated the microbial communities associated with the seagrass Enhalus acoroides throughout Singapore and Peninsular Malaysia. At each sampling location we collected 10 individual and unconnected plants. Each plant was subsequently broken down into leaves, roots, and rhizomes. In addition to living plant parts a sediment sample was taken in close proximity to each. Using high throughput 16S rRNA gene amplicon sequencing we characterised the bacterial communities associated with each plant part and the associated sediment sample. Results indicate geographic structuring of bacterial communities, with a significant pattern of distance decay suggesting dispersal limitation is a contributing factor to the differences we see in bacterial community structure. Bacterial communities can be further differentiated by the function of the collected sample (leaf, root, and rhizome), and we identified a number of microbial indicator species that are associated with each plant part. Further analysis revealed the presence of several microbial taxa that have previously been identified as indicators of “unhealthy” or “stressed” seagrass meadows. This study addresses a current scientific gap related to the characterisation of seagrass microbiomes, and provides a foundation on which future studies can build, particularly those in the Southeast Asian seagrass biodiversity hotspot. © Copyright © 2021 Rabbani, Yan, Lee, Ooi, Lee, Huang and Wainwright.