Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2012.04.056
Title: A β-sheet structure interacting peptide for intracellular protein delivery into human pluripotent stem cells and their derivatives
Authors: Lo, S.L.
Lua, S.
Song, J. 
Wang, S. 
Keywords: Peptide
Pluripotent stem cells
Protein delivery
Self-assembly
Issue Date: 11-May-2012
Source: Lo, S.L., Lua, S., Song, J., Wang, S. (2012-05-11). A β-sheet structure interacting peptide for intracellular protein delivery into human pluripotent stem cells and their derivatives. Biochemical and Biophysical Research Communications 421 (3) : 616-620. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2012.04.056
Abstract: The advance in stem cell research relies largely on the efficiency and biocompatibility of technologies used to manipulate stem cells. In our previous study, we had designed an amphipathic peptide RV24 that can deliver proteins into cancer cell lines efficiently without significant side effects. Encouraged by this observation, we moved forward to test whether RV24 could be used to deliver proteins into human embryonic stem cells and human induced pluripotent stem cells. RV24 successfully mediated protein delivery into these pluripotent stem cells, as well as their derivatives including neural stem cells and dendritic cells. Based on NMR studies and particle surface charge measurements, we proposed that hydrophobic domain of RV24 interacts with β-sheet structures of the proteins, followed by formation of " peptide cage" to facilitate delivery across cellular membrane. These findings suggest the feasibility of using amphipathic peptide to deliver functional proteins intracellularly for stem cell research. © 2012 Elsevier Inc.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/99990
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2012.04.056
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