Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(00)00376-0
Title: A zebrafish vitellogenin gene (vg3) encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene
Authors: Wang, H. 
Yan, T.
Tan, J.T.T.
Gong, Z. 
Keywords: EST
Fish
Lipovitellin
Polyserine
Teleost
Yolk
Issue Date: 3-Oct-2000
Source: Wang, H., Yan, T., Tan, J.T.T., Gong, Z. (2000-10-03). A zebrafish vitellogenin gene (vg3) encodes a novel vitellogenin without a phosvitin domain and may represent a primitive vertebrate vitellogenin gene. Gene 256 (1-2) : 303-310. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(00)00376-0
Abstract: By analysis of zebrafish EST (expressed sequence tag) clones from an adult cDNA library, we have identified 44 clones, about 11% of the adult EST clones, encoding vitellogenins. These vitellogenin EST clones have been derived from at least seven distinct vitellogenin genes. One of the largest vitellogenin cDNA clones, vg3, and its 5' extended clone isolated by 5' RACE (rapid amplification of cDNA ends)-PCR, have been sequenced completely. The deduced complete sequence includes a predicted mature vitellogenin of 1233 amino acids and a truncated signal peptide of 18 amino acids. Interestingly, the predicted vitellogenin has no polyserine phosvitin domain. The lack of the phosvitin domain was confirmed by isolation and sequencing of the vg3 genomic region. Phylogenetic analysis indicates that the phosvitinless vitellogenin is an intermediate between invertebrate vitellogenins and all known vertebrate vitellogenins, and thus may represent a primitive vertebrate vitellogenin. Like other vitellogenins in vertebrates, the phosvitinless vitellogenin is also synthesized mainly in the liver and weakly in the intestine. Copyright (C) 2000 Elsevier Science B.V.
Source Title: Gene
URI: http://scholarbank.nus.edu.sg/handle/10635/99988
ISSN: 03781119
DOI: 10.1016/S0378-1119(00)00376-0
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

99
checked on Feb 21, 2018

WEB OF SCIENCETM
Citations

91
checked on Jan 16, 2018

Page view(s)

17
checked on Feb 18, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.