Please use this identifier to cite or link to this item:
|Title:||A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis|
|Authors:||Yu, H.B. |
|Citation:||Yu, H.B., Rao, P.S.S., Lee, H.C., Vilches, S., Merino, S., Tomas, J.M., Leung, K.Y. (2004-03). A Type III Secretion System Is Required for Aeromonas hydrophila AH-1 Pathogenesis. Infection and Immunity 72 (3) : 1248-1256. ScholarBank@NUS Repository. https://doi.org/10.1128/IAI.72.3.1248-1256.2004|
|Abstract:||Aeromonas hydrophila is a gram-negative opportunistic pathogen in fish and humans. Many bacterial pathogens of animals and plants have been shown to inject anti-host virulence determinants into the hosts via a type III secretion system (TYSS). Degenerate primers based on lcrD family genes that are present in every known TTSS allowed us to locate the TTSS gene cluster in A. hydrophila AH-1. A series of genome walking steps helped in the identification of 25 open reading frames that encode proteins homologous to those in TYSSs in other bacteria. PCR-based analysis showed the presence of lcrD homologs (ascV) in all of the 33 strains of A. hydrophila isolated from various sources. Insertional inactivation of two of the TTSS genes (aopB and aopD) led to decreased cytotoxicity in carp epithelial cells, increased phagocytosis, and reduced virulence in blue gourami. These results show that a TTSS is required for A. hydrophila pathogenesis. This is the first report of sequencing and characterization of TTSS gene clusters from A. hydrophila. The TTSS identified here may help in developing suitable vaccines as well as in further understanding of the pathogenesis of A. hydrophila.|
|Source Title:||Infection and Immunity|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Nov 11, 2018
WEB OF SCIENCETM
checked on Oct 23, 2018
checked on Oct 26, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.