Please use this identifier to cite or link to this item: https://doi.org/10.1186/gb-2013-14-7-r69
Title: A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases
Authors: Lim, S.
Wang, Y.
Yu, X.
Huang, Y.
Featherstone, M.S.
Sampath, K. 
Issue Date: 1-Jul-2013
Source: Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., Sampath, K. (2013-07-01). A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases. Genome Biology 14 (7) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/gb-2013-14-7-r69
Abstract: Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes. © 2013 Lim et al.; licensee BioMed Central Ltd.
Source Title: Genome Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/99964
ISSN: 1474760X
DOI: 10.1186/gb-2013-14-7-r69
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