Please use this identifier to cite or link to this item: https://doi.org/10.1002/stem.1307
Title: A PRC2-dependent repressive role of PRDM14 in human embryonic stem cells and induced pluripotent stem cell reprogramming
Authors: Chan, Y.-S.
Göke, J.
Lu, X.
Venkatesan, N.
Feng, B.
Su, I.-H.
Ng, H.-H. 
Keywords: Embryonic stem cells
PRC2
PRDM14
Reprogramming
Issue Date: Apr-2013
Citation: Chan, Y.-S., Göke, J., Lu, X., Venkatesan, N., Feng, B., Su, I.-H., Ng, H.-H. (2013-04). A PRC2-dependent repressive role of PRDM14 in human embryonic stem cells and induced pluripotent stem cell reprogramming. Stem Cells 31 (4) : 682-692. ScholarBank@NUS Repository. https://doi.org/10.1002/stem.1307
Abstract: PRDM14 is an important determinant of the human embryonic stem cell (ESC) identity and works in concert with the core ESC regulators to activate pluripotencyassociated genes. PRDM14 has been previously reported to exhibit repressive activity in mouse ESCs and primordial germ cells; and while PRDM14 has been implicated to suppress differentiation genes in human ESCs, the exact mechanism of this repressive activity remains unknown. In this study, we provide evidence that PRDM14 is a direct repressor of developmental genes in human ESCs. PRDM14 binds to silenced genes in human ESCs and its global binding profile is enriched for the repressive trimethylation of histone H3 lysine 27 (H3K27me3) modification. Further investigation reveals that PRDM14 interacts directly with the chromatin regulator polycomb repressive complex 2 (PRC2) and PRC2 binding is detected at PRDM14-bound loci in human ESCs. Depletion of PRDM14 reduces PRC2 binding at these loci and the concomitant reduction of H3K27me3 modification. Using reporter assays, we demonstrate that gene loci bound by PRDM14 exhibit repressive activity that is dependent on both PRDM14 and PRC2. In reprogramming human fibroblasts into induced pluripotent stem cells (iPSCs), ectopically expressed PRDM14 can repress these developmental genes in fibroblasts. In addition, we show that PRDM14 recruits PRC2 to repress a key mesenchymal gene ZEB1, which enhances mesenchymal-to-epithelial transition in the initiation event of iPSC reprogramming. In summary, our study reveals a repressive role of PRDM14 in the maintenance and induction of pluripotency and identifies PRDM14 as a new regulator of PRC2. © AlphaMed Press.
Source Title: Stem Cells
URI: http://scholarbank.nus.edu.sg/handle/10635/99932
ISSN: 10665099
DOI: 10.1002/stem.1307
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