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|Title:||A comparison and optimization of methods and factors affecting the transformation of Escherichia coli|
|Citation:||Chan, W.-T., Verma, C.S., Lane, D.P., Gan, S.K.-E. (2013). A comparison and optimization of methods and factors affecting the transformation of Escherichia coli. Bioscience Reports 33 (6) : -. ScholarBank@NUS Repository. https://doi.org/10.1042/BSR20130098|
|Abstract:||DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for DH5α, XL-1 Blue and JM109 strains (P|
|Source Title:||Bioscience Reports|
|Appears in Collections:||Staff Publications|
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