Please use this identifier to cite or link to this item: https://doi.org/10.1016/0960-8524(95)00132-8
Title: Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes
Authors: Wang, W.J.
Powell, A.D. 
Oates, C.G.
Keywords: Amylase
Glucoamylase
Raw starch hydrolysis
Sage starch
Starch oligosaccharide profiles
Issue Date: Jan-1996
Citation: Wang, W.J., Powell, A.D., Oates, C.G. (1996-01). Sago starch as a biomass source: Raw sago starch hydrolysis by commercial enzymes. Bioresource Technology 55 (1) : 55-61. ScholarBank@NUS Repository. https://doi.org/10.1016/0960-8524(95)00132-8
Abstract: Raw sage starch and sage starch pretreated by heating at 60°C for 2 hours in sodium acetate buffer (pH 3.5) were hydrolysed using commercial glucoamylase-AMG (EC 3.2.1.3), α-amylases-BAN, Fungamyl and Termamyl (EC 3.2.1.1), debranching amylase-Promozyme (EC 3.2.1.41), and their mixtures in sodium acetate buffer, pH 5.0 at 35°C. Raw sage starch was a poor substrate for enzyme action compared to corn and tapioca starches tested under the same conditions, although pretreating the starch increased the extent and rate of hydrolysis. A strong synergism between glucoamylase and α-amylase on the hydrolysis of both untreated and pretreated sage starch was observed. The hydrolysis products were characterized by high-performance size-exclusion chromatography (HPSEC). The total carbohydrate concentration of hydrolysed sage starch decreased but the amylose and amylopectin ratios in the residues remained the same.
Source Title: Bioresource Technology
URI: http://scholarbank.nus.edu.sg/handle/10635/99744
ISSN: 09608524
DOI: 10.1016/0960-8524(95)00132-8
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